Western blot analysis of whole cell lysates, triton soluble, and desmosome-containing triton insoluble fraction showed that expression of Dsg2 was also elevated in Dsg3 ko cells compared to wt cells, in line with the observation that pemphigus autoantibodies induce upregulation of Dsg2 in human epidermis (Figure 3B). Here, DSG3 is linked to pemphigus.