FOXP3 and neoplasm: Single immune cells were detected with trained algorithms in annotated tumor, invasive margin and normal colon ROI and cell densities of CD11b+CD14+, CD11b+CD15+ and ARG1+ myeloid cells, and CD8+Ki67+/–, CD8+Ki67+/– and FOXP3+ T cells were computed respectively (Figures 2A, B).