We first investigated DNA methylation patterns in KCNN3, MUC4, GALR1, SOD3, and WDR45B genes that are implicated in auditory functioning and hearing loss across the two breaching protocols in the current FLW and previously published studies, and found that the methylation patterns of the DMRs in these loci did not replicate. The gene discussed is KCNN3; the disease is hearing loss disorder.