Middle cerebral artery occlusion (MCAO) was performed to produce brain ischemia, a maneuver that induces cerebral edema, in wild type (WT), WNK3–/–, and SPAK–/– mice (Begum et al., 2015; Zhang et al., 2016; Zhao et al., 2017). Here, WNK3 is linked to brain ischemia.