No upregulation of NCC phosphorylation is observed in WNK4–/– mice (Castaneda-Bueno et al., 2014; Yang et al., 2018) and, consequently, mice develop severe hypokalemia when maintained on a low K+ diet (Castaneda-Bueno et al., 2014). The gene discussed is SLC12A3; the disease is Hypokalemia.