Using multiple immunofluorescence approaches to tissues affected by different durations of focal cerebral ischemia, a spatio-temporal characterization of collagen IV as the major part of the basement membrane (Thomsen et al., 2017; Gatseva et al., 2019), and fibronectin as a major component of the ECM (Singh et al., 2010; Mezzenga and Mitsi, 2019) was performed. Here, FN1 is linked to brain ischemia.