Moreover, the extent of MI was determined by Evans blue and TTC staining, which illustrated similar ratios of area at risk (AAR) to left ventricular area in the MI/R rats treated with oe-USP22, oe-USP22 + sh-SLC7A11, and oe-USP22 + SIRT1. This evidence concerns the gene SIRT1 and myocardial infarction.