Consistent with the transcriptome analysis result, the qPCR analysis results showed that the Nox1 mRNA expression in the colons of IL-4Rα-/- colitis mice was significantly higher than that in the colons of WT colitis mice (Figure 5A; 0.21 ± 0.04 in WT colitis, 2.00 ± 0.26 in IL-4Rα-/- colitis; p < 0.05). This evidence concerns the gene IL4R and colitis.