MS-HRM analyses showed that MLH1 methylation levels were very low in peripheral blood (0.64 ± 0.13%), adjacent tumor tissue (0.40 ± 0.19%), and in thymomas (0.19 ± 0.14%), and no overall difference among the three tissues was observed (p = 0.08; Figure 2A). This evidence concerns the gene MLH1 and thymoma.