To prevent donor-associated variables (sex, age, postmortem delay, tissue quality, etc.)affecting microglia clustering, each donor was analyzed individually: 2,881 cells were analyzed from donor 1: AD, female, 81 years, LPS tissue with high Aβ burden, and modest but visible PHF-tau protein (donor #2018-135, Supplementary Table S2), and 3,079 single cells were analyzed from donor 2: MCI donor, female, 77 years old, LPS tissue with moderate Aβ pathology, and high levels of PHF-tau deposits (donor #2019-010, Supplementary Table S2). This evidence concerns the gene MAPT and Alzheimer disease.