Impaired autophagic pathway confirmed also analysis of the late autophagic vesicles; immunostaining with lysosome-associated membrane protein 1 (LAMP-1) antibody revealed enlarged and annular LAMP-1-positive organelles in AMD RPE as opposed to the smaller discrete puncta observed in normal RPE (Golestaneh et al., 2017[40]). This evidence concerns the gene LAMP1 and age-related macular degeneration.