CD4 and Sepsis: Thus, to address the intrinsic and extrinsic impact of sepsis on priming of CD4 T cells, 2D2 cell number, proliferation, as assessed by recent proliferation marker Ki67 (Miller et al., 2018), and apoptosis, as assessed by presence of active caspase 3/7 (FLICA+) and membrane depolarization (PI+), were evaluated in the iLN 7 days post-EAE induction (Figure 6a,b).