In order to investigate the synergistic effect of MEX3A and CDK6 on the development of ESCC, Eca-109 cells with MEX3A overexpression (MEX3A group), CDK6 knockdown (shCDK6 group) and simultaneous MEX3A overexpression and CDK6 knockdown (MEX3A+shCDK6 group) were constructed and verified using abovementioned method (Supplementary Figure 4A). This evidence concerns the gene CDK6 and esophageal squamous cell carcinoma.