This study was performed to inspect the prevalence, antibiogram, PCR detection of virulence genes (tsh, phoA, hly, eaeA, sta, and lt) as well as the antibiotic-resistance genes (blaTEM, blaKPC, and blaCTX) of E. coli isolated from secondary bacterial infections following FMD-outbreak in cattle. The gene discussed is EMD; the disease is bacterial infectious disease.