We simultaneously analyzed the phosphorylation/activated state of STAT3, Akt, Erk1/2, and p38 in the distal colon and tumor tissues from IRF3+/+, IRF3–/–, IRF3fl/fl, and IRF3fl/flVillincre mice following AOM/DSS treatment. The gene discussed is AKT1; the disease is infectious otitis media.