In some experiments, non-transfected A549 cells were treated with human recombinant (ImmunePharma srl., Italy) of the large subunit of caspase-4 (100 ng/ml) and co-cultered with peripheral blood mononuclear cells (PBMCs) obtained by NSCLC patients (ratio 1:5). Here, CASP4 is linked to non-small cell lung carcinoma.