VIM gene had a significantly higher expression in the U251 and U87 cell lines’ sEVs compared to the NCH644 GBM stem cell line sEVs (p < 0.0001 and p = 0.0003, respectively) and normal astrocyte sEVs (p < 0.0001), while the NAP1L1 gene expression was significantly higher in sEVs from U251 and NCH644 compared to those of astrocytes (p = 0.0002 and p = 0.0300, respectively). Here, NAP1L1 is linked to glioblastoma.