Finally, we investigated whether the target genes of the selected miRNAs—NAP1L1, FREM2, SPRY1, VIM, and NCL—that have been associated with GBM in our previous studies [6,19,20,21] were also present in the sEVs of different cell types and showed any particular expression pattern in GBM-isolated sEVs compared to normal astrocyte cells. This evidence concerns the gene NUCLEOLIN and glioblastoma.