Lipid accumulation stimulates hepatic inflammatory cells and hepatocytes to secrete inflammatory cytokines and mediators, such as tumor necrosis factor-α (TNFα), interleukin (IL)-6, IL-1β, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX2), which is usually correlated with the severity of NAFLD [36,37]. This evidence concerns the gene NOS2 and metabolic dysfunction-associated steatotic liver disease.