Multiple compressions and Tukey’s post hoc analysis showed that compared to the sham group (0.45 ± 0.03), the stroke group (0.33 ± 0.03, p = 0.002) and BF+stroke group (0.25 ± 0.04, p < 0.001) had lower levels of claudin-5 in hippocampi ipsilateral to the stroke injury. Here, CLDN5 is linked to stroke disorder.