In terms of lipid oxidative genes, including HMGCS2, CPT2 and CPT1A, no significant changes were observed (Figure 2C), indicating that lipid oxidation might not be responsible for the increased lipid deposition in liver, at least not in the early stages of hyperglycaemia or hyperlipidemia induced NAFLD. The gene discussed is CPT2; the disease is metabolic dysfunction-associated steatotic liver disease.