However, in our study, we revealed the transcriptional regulation of DEPTOR by the tumor suppressor, p53, in various types of cancer cells, including breast cancer (MCF7), prostate cancer (LNCap), lung cancer (A549), colon cancer (HCT116), and osteosarcoma (U2OS and SJSA) cells, as well as in mouse tissues under unstressed conditions in vivo (Fig. 1 and S1), indicating a key role of p53 in regulating DEPTOR-mediated physiological processes. This evidence concerns the gene DEPTOR and osteosarcoma.