To further demonstrate the positive correlation between DEPTOR expression and p53 activity, we depleted p53 by CRISPR-Cas9 technology in U2OS osteosarcoma cells harboring wild-type p53 and found an obvious decrease in the protein and mRNA levels of DEPTOR in two individual p53-null clones (Fig. 1e, f). This evidence concerns the gene TP53 and osteosarcoma.