To further explore the function of endogenous ENO1 in HCC cells, we used lentivirus technology to induce stable ENO1 knockdown in HCCLM3 cells and ENO1 overexpression in MHCC97L and HepG2 cells, which were used for loss-of-function and gain-of-function studies (Supplementary Fig. 5A, B). Here, ENO1 is linked to hepatocellular carcinoma.