Second, the western blot results showed that ENO1 overexpression significantly upregulated integrin α6 and β4 expression in MHCC97L and HepG2 cells (Supplementary Fig. 6C), suggesting that ENO1 can regulate integrin α6β4 complexes in HCC cells to promote HCC progression. This evidence concerns the gene ENO1 and hepatocellular carcinoma.