Therefore, considering previous literature, our results have first shown the enhanced number of CD4+ cells and Tregs in splenocytes of mice (with DSS-induced colitis) (Figure 8A); second, Th1 cytokine (i.e., IFNγ) was found to be increased only in the DSS group and its levels decreased by Rg3-RGE + PT treatment (Figure 9A), whereas, Th2 cytokine, (i.e., IL-4) was remarkably enhanced by treatment with the mixture (Figure 9B). Here, CD4 is linked to colitis.