Ma and Liu reported that treatment of obesity-prone ARK/J mice with PXR ligand pregnenolone 16α-carbonitrile (PCN) inhibited weight gain under HFD and at the final time point of seven weeks, the weight of the PCN-treated mice was similar to the chow-fed mice [20]. This evidence concerns the gene NR1I2 and obesity due to melanocortin 4 receptor deficiency.