Moreover, treated CT26-implanted mice were able to survive tumor re-challenge, and mice implanted with bi-lateral A20 tumors consistently cleared both tumors following unilateral injection with VG161 while succumbing to tumor burden after treatment with a variant of VG161 lacking IL-12, IL-15/IL-15RA, and the PD-L1 blocker (VG160). This evidence concerns the gene DDX53 and neoplasm.