Alteration in HH signaling pathway promotes GLI translocation into the nucleus leading to overactivation of several target genes which regulate cell differentiation (INSM1, SOX2, OCT4 and NANOG) [5–7], proliferation (c-MYC and n-MYC) [8, 9], apoptosis (BCL2, CASPASE 3, BAX, CASPASE 9 and BAK) [8–12], cell cycle (CCND1 and P21) [8, 11, 13–15], DNA damage (RAD51 and TP53) [15, 16], angiogenesis (c-MET, VEGFR2) [11, 17] and adhesion (N-CADHERIN, E-CADHERIN and SNAIL 1) [13, 18, 19] contributing to the pathogenesis of cancer. The gene discussed is GLI1; the disease is cancer.