Similar to the CCl4 model, Treg cells significantly were increased in the liver by feeding the diet (Supplementary Figures 1B,C) and Treg depletion aggravated liver fibrosis assessed by liver fibrotic regions and expression levels of Col1a1 and Acta2 (α-SMA) (Supplementary Figures 1D,E). Here, ACTA1 is linked to Hepatic fibrosis.