This IL-12 secreting HSV was combined with both anti-CTLA-4 and anti-PD-1 checkpoint blockade for the treatment of two murine GBM models, this triple therapy reduced the number of regulatory T cells present within tumors and increased the influx of immune supporting M1 macrophages resulting in the complete cure of these mice (200). The gene discussed is CTLA4; the disease is glioblastoma.