In Esophageal Squamous Cell Carcinoma (ESCC), aberrant hypermethylation of ZNF582 and PAX1 have been demonstrated using quantitative methylation-specific PCR with levels at 21% versus 0% for tumor and peri-tumor normal tissues, respectively (Huang et al., 2017). This evidence concerns the gene ZNF582 and esophageal squamous cell carcinoma.