TNF and Stroke: Furthermore, in the ki20227-treated stroke mice, the mRNA expression levels of pro-inflammatory factors such as iNOS and BCL-2 (Figure 3B) and the cytokine-related gene TNF-α were significantly increased compared with those in the vehicle-treated stroke mice on day 3 after reperfusion (Figure 3A), but the mRNA expression levels of anti-inflammatory factors such as TGF-β were significantly decreased in the ki20227-treated stroke mice compared with the vehicle-treated stroke mice on day 3 after reperfusion (Figure 3A).