To confirm the effects of PBRM1 on mast cell recruitment, we performed an in vitro Transwell migration assays using 5×104 HMC-1 cells in the upper chamber and PBRM1-knockdown ccRCC cells (786-O and Caki-1, Figure 5A, 4B) or conditioned media from these cells in the lower chamber. This evidence concerns the gene PBRM1 and nonpapillary renal cell carcinoma.