Pathway analysis of the dark-orange and white modules showed that they contained genes played a role in the Notch, MAPK (mitogen-activated protein kinase), and TGF-β (transforming growth factor-β) signaling pathways, tyrosine kinases, and extracellular matrix organization (Figure 3D, 3E), this suggests that PBRM1MUT enhanced ccRCC progression though the Notch, MAPK, and TGF-β pathways. The gene discussed is TGFB1; the disease is nonpapillary renal cell carcinoma.