A luciferase assay conducted 24 h postinfection (hpi) showed a 13-fold increase of HIV-1 infection in SAMHD1 KO cells compared to control cells, which were reduced 1.5-fold or 1.8-fold by 5Z or Takinib treatment, respectively (Fig. 5A). This evidence concerns the gene SAMHD1 and HIV-1 infection.