H2BC21 and systemic lupus erythematosus: The highest colocalization between ISG15 and H2B was found in spontaneous NETs of patients with SLE followed by the colocalization in LPS induced NETs from SLE patients, and as expected the lowest was observed in NETs from healthy controls, with a significant difference between lupus NETs and healthy controls samples (0.75 [0.73–0.86] vs 0.21 [0.18–0.23] p < 0.05) (Fig. 3e).