In contrast, alterations in the known breast cancer biomarkers HER2 (ERBB2) (Fig. 5b), ER (ESR1) (Fig. 5c), and PIK3CA (Fig. 5d) showed no correlation with overall survival in the same invasive breast cancer data set we examined for BRF2 alterations. The gene discussed is PIK3CA; the disease is invasive breast carcinoma.