In conclusion, our present study is the first to uncover the upregulation of circSEMA5A in BC, disclosing that circSEMA5A facilitates bladder cancer progression by mediating miR-330-5p/ENO1 signaling and upregulating SEMA5A expression, indicating the potential value of circSEMA5A as biomarkers and therapeutic target for BC. Here, ENO1 is linked to breast cancer.