In addition, hypermethylated NEUROG1, RASSF1A, RASSF2A, SDC2, SEPT9, TAC1, and THBD genes were detected in early stage CRC, hypermethylation of ALX4, FBN2, HLTF, P16, TMEFF1, and VIM genes was associated with poor prognosis, and hypermethylated P16 and TFPI2 genes were related to recurrence of CRC [14]. This evidence concerns the gene ALX4 and colorectal carcinoma.