In addition, the proliferation and migration of SW480-GFRA1 and HCT116-GFRA1 hypomethylated cells were reduced by treatment with the inhibitors, MK-2206 2HCl and SP600125 (Fig 6G and 6H), suggesting that the promotion of GFRA1 hypomethylation in the CRC cell proliferation and invasion can be blocked by p-AKT and p-c-Jun inhibitors. Here, AKT1 is linked to colorectal carcinoma.