MAPK8 and colorectal carcinoma: To determine whether GFRA1 demethylation induces EMT by promoting AKT and c-Jun phosphorylation in CRC, the AKT inhibitor MK-2206 2HCl and JNK inhibitor SP600125 were added at a concentration of 2 μM for 48 h to the GFRA1 targeted demethylation CRC cells.