To further determine the role of IGF1R in the growth of human OPC tumor cells in vivo, we acutely transfected patient‐derived glioma cells with Piggybac (PB) transposon vectors encoding multiplex miRNAs against different sites of IGF1R (Mir‐IGF1R) or against Luciferase (Mir‐Luc) (Figure 6J,K). Here, IGF1R is linked to central nervous system cancer.