The aim of thepresent work is to use the GLAD-PCR assay to detect the aberrantly methylatedR(5mC)GY sites in the regulatory regions of tumor suppressor genes(brinp1, bves, cacna2d3,cdh11, cpeb1, epha7,fgf2, galr1, gata4,hopx, hs3st2, irx1,lrrc3b, pcdh10, rprm,runx3, sfrp2, sox17,tcf21, tfpi2, wnt5a,zfp82, and znf331) in DNA samples obtainedfrom gastric cancer (GC) tissues. This evidence concerns the gene RUNX3 and gastric cancer.