In contrast to these studies which utilized Sox9, Krt19 and Spp1 as a driver of LPC specific labeling, we used Prom1-Cre to determine the fate of LPCs/DRPs and TICs in liver tumor development based on their shared expression of Prom1. Our results revealed that PROM1+ cells which were derived in part from hepatocytes in AH, gave rise to both DRPs and liver tumor cells in the DEN-WAD mouse model. This evidence concerns the gene SPP1 and acrofacial dysostosis, Weyers type.