Of interest, three of the 4 top genes expressed in this subpopulation (Fig. 4e), are metabolic genes: Gulo, beta-catenin responsive gene which encodes the limiting enzyme in the final step in ascorbic acid biosynthesis43, Cyp2c70, a key enzyme in generation of the murine specific bile acid, a-muricholic acid, or Elovl2, an enzyme involved in elongation of long chain w-3 and w-6 fatty acids which was shown to confer stemness of glioma cells44. Here, GULOP is linked to central nervous system cancer.