UBP1 and parasitic infectious disease: We therefore developed a modified in vivo RSA, where we injected wild-type, UBP-1 V2721F, M488I, and R551T parasites back into mice 24 h after dimethyl sulfoxide (DMSO) or DHA exposure in the RSA as described above and then assessed viability by quantifying in vivo parasitemia on day 4.