Apart from insulin-treated diabetes mellitus type 2 (5/6; 83%), further risk factors associated with non-healing lesions involved arterial hypertension (5/6, 83%), Charcot foot or foot deformity (3/6; 50%), renal impairment or chronic kidney disease (3/6; 50%), coronary heart disease or ischemic cardiomyopathy (50%), atrial fibrillation (50%), dyslipidaemia (50%) and critical limb ischemia or peripheral artery disease (2/6; 33%). The gene discussed is INS; the disease is chronic kidney disease.