The reference-free diagnostic panel used the ratio of relative expression of three mRNAs that are overexpressed (FOLH1, XBP1 and HPN) to five mRNAs that are underexpressed (ITSN1, GSTM4, LTBP4, NELL2, and CFD) in prostate cancer compared to normal tissue. This evidence concerns the gene ITSN1 and prostate cancer.