We analyzed the expression of the primary adipogenic (ADIPOQ, LPL, PPAR-γ and Glut4) and ECM-remodeling (collagen, FN, LN, ITAGA5, MMP2, 9 and 11) markers at the transcriptional level and by immunohistochemistry in 3D spheroids derived from ASCs from both lipedema and healthy individuals. The gene discussed is MMP2; the disease is Lipedema.