The data demonstrate that both undifferentiated and adipogenic-differentiated spheroids express fibronectin, collagen 1A1 and collagen 6 isoforms (A1-3); although there was no difference in the expression of ECM components between lipedema and healthy spheroids, a decrease in gene expression for MMPs, in particular MMP11, in lipedema spheroids was noted. This evidence concerns the gene FN1 and Lipedema.