ACTA1 and glomerulosclerosis: We also detected less glomerulosclerosis and tubulointerstitial fibrosis (assessed by collagen IV immunofluorescence analysis and by Picrosirius red staining) in the kidneys from high-fat diet–fed Stk25-KO versus WT mice, in parallel with suppressed arteriolar hyalinosis (quantified by α-SMA immunostaining) (Figure 5, D–F).