However, when taking into account the micro-regional variations using the square regions, the IQR of region-based antibody penetration ratio across the tumor section was significantly lower in patients receiving a loading dose compared with the patients in the non-LD group (Fig. 5c) and the Pearson’s correlation coefficient between the fluorescence and the EGFR expression of the square regions across each tumor was significantly higher in the LD than the non-LD dose group (Fig. 5d). This evidence concerns the gene EGFR and neoplasm.