We performed western blotting analysis of the EZH2 expression in an immortalized human liver cell HL-7702 and a panel of HCC cell lines, indicating that the expression level of EZH2 was higher in huh7 and hepG2 cell lines compared to the rest of HCC cell lines and HL-7702 (Fig. 3a). This evidence concerns the gene EZH2 and hepatocellular carcinoma.