NOTCH1 and neoplasm: Trp53 mutations positively correlated with high grade tumours (p = 0.05160) but no other parameters, while Notch1 mutations correlated significantly with an increased total immune cell infiltrate (CD45+, p = 0.00069), T-cell infiltrate (CD3+, p = 0.02019) and high tumour grade (p = 0.01288)(Fig. 5e, Supplementary Data 6).