H3K27Ac ChIP sequencing of nine of 12 of the nanopore long read sequenced samples performed in parallel revealed acetylation of one or both ABCB1 promoters in every sample, demonstrating that high level ABCB1 expression in relapsed AML is driven by native promoters (Fig. 2f), at least in the samples evaluated. The gene discussed is ABCB1; the disease is acute myeloid leukemia.