In good agreement, the overexpression of TSPAN1 in human cancer vs. adjacent non-cancerous tissue has been widely documented in cholangiocarcinoma [31,47,48], skin squamous cell carcinoma [36,49], esophageal carcinoma [34], ovarian carcinomas [50], prostate cancer [26], pancreatic cancer [35,37] and gastric carcinoma [51,52]. This evidence concerns the gene TSPAN1 and cholangiocarcinoma.