In order to verify the proteomic results, TSPAN1 expression was analyzed by Western blot in the aforementioned HNSCC cell lines confirming that TSPAN1 was upregulated in CCL-138-R, JHU029-R and HTB-43-R cells compared to their respective control cells (Figure 1C, Figure S8). This evidence concerns the gene TSPAN1 and head and neck squamous cell carcinoma.