In testosterone-treated TM4 and PSC cells the expression of Hes1/HES1 was not affected by HF (Figure 10a,c,d,f), whereas Bic inhibited the effect of testosterone on Hes1 mRNA and HES1 protein expression in all cellular models (p < 0.01, p < 0.001) (Figure 10a–f). Here, HES1 is linked to hydrops fetalis.