Prognostic analysis of 109 differentially expressed genes was performed by GEPIA online analysis tool. The results showed that the expression levels of four of the genes were significantly correlated with the overall survival of pancreatic cancer patients in the TCGA database, namely SCG5, CRYBA2, CPE and CHGB (Figure 5A). Using the data of pancreatic cancer patients in the GEO database to verify the relationship between the expression of four differential genes and prognosis, the KM survival curve was drawn (Figure 5B), and the results were consistent with the TCGA data analysis. The gene discussed is CRYBA2; the disease is familial pancreatic carcinoma.